Title: High- and Super-Resolution Fluorescence Microscopy

Speaker: Dr. J?rg Enderlein

Time: August 8 2016 (Mon) 9:30-10:30

Venue: Room 208,Jinguang Life Sciences Building

Abstract:

<span "="">The presentation provides a general introduction into the field of high and superresolution fluorescence microscopy, starting with the physical origin of the classical resolution limit. It continuous with an overview of the different techniques which have been invented over the last 20 years for pushing the limits of the diffraction-limited resolution and even going beyond these limits. In particular, I will discuss in detail Structured Illumination Microscopy (SIM), and the recently introduced Image Scanning Microscopy (ISM). Next, the presentation turns to the recently developed “true” superresolution microscopy approaches, which indeed circumvent the classical diffraction-related resolution limit. The last part of the talk will focus on several lesser known techniques such as Stochastic Optical Fluctuation Imaging (SOFI) or Metal Induced Energy Transfer (MIET) Imaging, which are particularly interesting methods due to their exceptional simplicity and broad applicability. Special emphasis will be put on bridging the gap between the physical principles behind all these methods, their practicability, and the gain in information when applied to bioimaging.

Host:Peng Xi,Chen Zhang